Junxing Chen

Laboratory Technician/Manager, Department of Biochemistry

Laboratory Scientist, GenuIN Biotech LLC, Blacksburg, VA 

Laboratory Assistant, GenuIN Biotech LLC, Blacksburg, VA

Assistant in the Office/laboratory Assistant, American & Efird Thread LLC.

Teaching Assistant, Department of Biochemistry BCHM 4124, Spring 2021

GECX-RNA offered an innovative method to study the protein-RNA interaction in vivo.

Researchers can identify a specific binding site on nucleotide resolution without using

antibodies based on GRIP-Seq. In this technique, a reader protein with unnatural amino

acid will crosslink with specific sites on mRNA. After proteinase K treatment, there would

be residues on the binding sites which will terminate reverse-transcription. And all

binding sites crosslinked with Protein-UAA would be captured and identified.

However, GRIP-Seq can only locate protein binding sites on fragment mRNA, The

detection of binding site can not be located accurately in isoform RNA. By identifying the

mutation of nucleotides, the binding site can be located in the full length of mRNA

without effect of isoform RNA.